H2AFX antibody (N-Term)

Catalog No. ABIN2787619

The Rabbit Polyclonal anti-H2AFX antibody has been validated for WB. It is suitable to detect H2AFX in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Zebrafish (Danio rerio) and Horse. There is 1 publication available.

Quick Overview for H2AFX antibody (N-Term) (ABIN2787619)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Zebrafish (Danio rerio), Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-H2AFX Antibody

Binding Specificity: N-Term

Sequence: SGRGKTGGKA RAKAKSRSSR AGLQFPVGRV HRLLRKGHYA ERVGAGAPVY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against H2AFX. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human H2AFX

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 143 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-H2AFX antibody (ABIN2787619)

Wen, Zhu, Zhang, Keum, Zykova, Yao, Peng, Zheng, Cho, Ma, Bode, Dong: "MST1 promotes apoptosis through phosphorylation of histone H2AX." in: The Journal of biological chemistry, Vol. 285, Issue 50, pp. 39108-16, (2010) (PubMed).

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2AFX

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H2AFX is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: H2A.X, H2A/X, H2AX
Protein Interaction Partner: UBC, HIST1H3A, USP17L2, UBD, RNF8, DDX21, XRCC6, NBN, NCL, HIST1H3B, ESR1, CENPA, TP53BP1, TOPORS, MDC1, BRCA1, ATR, ATM, PRKDC, CBX5, TRIM28, EGFR, BMI1, HIST2H3C, PML, XRCC5, UBB, TERF2, RNF2, SIRT7, CSNK2A1, ARRB2, ARRB1, PAXIP1, MRE11A, ATF2, MCPH1, C
Protein Size: 143

Molecular Weight: 16 kDa

Gene ID: 3014

NCBI Accession: NM_002105, NP_002096

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus