H2AFX antibody (Middle Region)

Catalog No. ABIN2787620

This Rabbit Polyclonal antibody specifically detects H2AFX in WB, IHC and IF. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog and Guinea Pig.

Quick Overview for H2AFX antibody (Middle Region) (ABIN2787620)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-H2AFX Antibody

Binding Specificity: Middle Region

Sequence: ELNKLLGGVT IAQGGVLPNI QAVLLPKKTS ATVGPKAPSG GKKATQASQE

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Human: 100%, Mouse: 79%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against H2AFX. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human H2AFX

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 143 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2AFX

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA
Alias Symbols: H2A.X, H2A/X, H2AX
Protein Interaction Partner: UBC, HIST1H3A, USP17L2, UBD, RNF8, DDX21, XRCC6, NBN, NCL, HIST1H3B, ESR1, CENPA, TP53BP1, TOPORS, MDC1, BRCA1, ATR, ATM, PRKDC, CBX5, TRIM28, EGFR, BMI1, HIST2H3C, PML, XRCC5, UBB, TERF2, RNF2, SIRT7, CSNK2A1, ARRB2, ARRB1, PAXIP1, MRE11A, ATF2, MCPH1, C
Protein Size: 143

Molecular Weight: 16 kDa

Gene ID: 3014

NCBI Accession: NM_002105, NP_002096

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus