H2AFX antibody (Middle Region)
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- Target See all H2AFX Antibodies
- H2AFX (H2A Histone Family, Member X (H2AFX))
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Binding Specificity
- Middle Region
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Reactivity
- Human, Mouse, Rat, Cow, Dog, Guinea Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This H2AFX antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Sequence
- ELNKLLGGVT IAQGGVLPNI QAVLLPKKTS ATVGPKAPSG GKKATQASQE
- Predicted Reactivity
- Cow: 93%, Dog: 93%, Guinea Pig: 93%, Human: 100%, Mouse: 79%, Rat: 86%
- Characteristics
- This is a rabbit polyclonal antibody against H2AFX. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of human H2AFX
- Top Product
- Discover our top product H2AFX Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 143 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- H2AFX (H2A Histone Family, Member X (H2AFX))
- Alternative Name
- H2AFX (H2AFX Products)
- Synonyms
- H2A.X antibody, H2A/X antibody, H2AX antibody, AW228881 antibody, H2ax antibody, Hist5-2ax antibody, gammaH2ax antibody, zgc:56329 antibody, h2a.x antibody, h2a/x antibody, h2ax antibody, RGD1566119 antibody, h2a antibody, h2afx antibody, H2A histone family member X antibody, H2A histone family, member X antibody, H2A histone family member X L homeolog antibody, histone cluster 1, H2ah antibody, histone cluster 2, H2ab S homeolog antibody, histone H2AX antibody, H2AFX antibody, H2afx antibody, h2afx antibody, h2afx.L antibody, HIST1H2AH antibody, hist2h2ab.S antibody, LOC100522201 antibody, LOC100720536 antibody
- Background
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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA
Alias Symbols: H2A.X, H2A/X, H2AX
Protein Interaction Partner: UBC, HIST1H3A, USP17L2, UBD, RNF8, DDX21, XRCC6, NBN, NCL, HIST1H3B, ESR1, CENPA, TP53BP1, TOPORS, MDC1, BRCA1, ATR, ATM, PRKDC, CBX5, TRIM28, EGFR, BMI1, HIST2H3C, PML, XRCC5, UBB, TERF2, RNF2, SIRT7, CSNK2A1, ARRB2, ARRB1, PAXIP1, MRE11A, ATF2, MCPH1, C
Protein Size: 143 - Molecular Weight
- 16 kDa
- Gene ID
- 3014
- NCBI Accession
- NM_002105, NP_002096
- UniProt
- P16104
- Pathways
- Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
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