CAPZA3 antibody (N-Term)

Catalog No. ABIN2787761

The Rabbit Polyclonal anti-CAPZA3 antibody is suitable to detect CAPZA3 in samples from Human, Mouse, Rat, Dog, Guinea Pig and Rabbit. It has been validated for WB.

Quick Overview for CAPZA3 antibody (N-Term) (ABIN2787761)

Target: CAPZA3 (Capping Protein (Actin Filament) Muscle Z-Line, alpha 3 (CAPZA3))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAPZA3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CAPZA3 Antibody

Binding Specificity: N-Term

Purpose: CAPZA3 Antibody - N-terminal region

Sequence: MTLSVLSRKD KERVIRRLLL QAPPGEFVNA FDDLCLLIRD EKLMHHQGEC

Predicted Reactivity: Dog: 92%, Guinea Pig: 92%, Human: 100%, Mouse: 85%, Rabbit: 92%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against CAPZA3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CAPZA3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58604-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CAPZA3

Target: CAPZA3 (Capping Protein (Actin Filament) Muscle Z-Line, alpha 3 (CAPZA3))

Alternative Name: CAPZA3

Background:

Targeted Subunit: alpha-3

Background Information: F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. CAPZA3 may play a role in the morphogenesis of spermatid.This gene encodes an actin capping protein, one of the F-actin capping protein alpha subunit family. The encoded protein is predominantly localized to the neck region of ejaculated sperm, other immunohistochemical signals were found in the tail and postacrosomal regions. The encoded protein may also form heterodimers of alpha and beta subunits. This protein may be important in determining sperm architecture and male fertility.

Gene Name: Capping protein (actin filament) muscle Z-line, alpha 3

Alternative Symbols: Gsg3, CAPPA3, HEL-S-86

Protein Name: F-actin-capping protein subunit alpha-3

Molecular Weight: 33kDa

Gene ID: 93661

NCBI Accession: NP_201585

UniProt: Q96KX2

Pathways: Regulation of Actin Filament Polymerization