WDR77 antibody (N-Term)

Catalog No. ABIN2787792

The Rabbit Polyclonal anti-WDR77 antibody (ABIN2787792) specifically detects WDR77 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig and Rabbit samples.

Quick Overview for WDR77 antibody (N-Term) (ABIN2787792)

Target: WDR77 (WD Repeat Domain 77 (WDR77))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WDR77 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-WDR77 Antibody

Binding Specificity: N-Term

Purpose: WDR77 Antibody - N-terminal region

Sequence: MRKETPPPLV PPAAREWNLP PNAPACMERQ LEAARYRSDG ALLLGASSLS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against WDR77. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human WDR77

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58692-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for WDR77

Target: WDR77 (WD Repeat Domain 77 (WDR77))

Alternative Name: WDR77

Background:

Background Information: WDR77 is the non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. WDR77 might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

Gene Name: WD repeat domain 77

Alternative Symbols: p44, MEP50, MEP-50, HKMT1069, Nbla10071, p44/Mep50

Protein Name: Methylosome protein 50

Molecular Weight: 38kDa

Gene ID: 79084

NCBI Accession: NP_077007

UniProt: Q9BQA1

Pathways: Ribonucleoprotein Complex Subunit Organization