Bcl-2 antibody (Middle Region)

Catalog No. ABIN2787860

The Rabbit Polyclonal anti-Bcl-2 antibody has been validated for WB. It is suitable to detect Bcl-2 in samples from Human, Mouse, Rat, Pig, Rabbit, Sheep, Dog, Cow, Guinea Pig and Horse.

Quick Overview for Bcl-2 antibody (Middle Region) (ABIN2787860)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Sheep, Dog, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Bcl-2 Antibody

Binding Specificity: Middle Region

Sequence: ALSPVPPVVH LTLRQAGDDF SRRYRRDFAE MSSQLHLTPF TARGRFATVV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against BCL2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human BCL2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 239 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: BCL2

Background: This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Alias Symbols: Bcl-2, PPP1R50
Protein Interaction Partner: KEAP1, SQSTM1, BECN1, UBC, TP53BP2, BID, BCL2L1, BCL2, BAX, BAD, ATP2A3, NR4A1, KRAS, PPID, MDM4, BNIP3, BFAR, CDK1, BAG3, RAF1, IKBKG, TP53, PPP3CC, FKBP8, MAPK8, IKBKB, HSP90AA1, HIF1A, CRYAB, CHUK, PML, BRCA1, AMBRA1, ATG12, BIK, CISD2, MEX3D, PPP1CC,
Protein Size: 239

Molecular Weight: 26 kDa

Gene ID: 596

NCBI Accession: NM_000633, NP_000624

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling