CD22 antibody (C-Term)

Catalog No. ABIN2788240

This Rabbit Polyclonal antibody specifically detects CD22 in WB. It exhibits reactivity toward Human and Horse.

Quick Overview for CD22 antibody (C-Term) (ABIN2788240)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD22 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD22 Antibody

Binding Specificity: C-Term

Purpose: CD22 Antibody - C-terminal region

Sequence: YSALHKRQVG DYENVIPDFP EDEGIHYSEL IQFGVGERPQ AQENVDYVIL

Predicted Reactivity: Horse: 79%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against CD22. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CD22

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP59862-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background:

Background Information: CD22 mediates B-cell B-cell interactions. CD22 may be involved in the localization of B-cells in lymphoid tissues. CD22 binds sialylated glycoproteins, one of which is CD45. CD22 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response CD22 seems to be involved in regulation of B-cell antigen receptor signaling. CD22 plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Gene Name: CD22 Molecule

Alternative Symbols: SIGLEC2, SIGLEC-2

Protein Name: B-cell receptor CD22

Molecular Weight: 95kDa

Gene ID: 933

NCBI Accession: NP_001762

UniProt: P20273