COL4a3 antibody (Middle Region)

Catalog No. ABIN2788301

The Rabbit Polyclonal anti-COL4a3 antibody (ABIN2788301) specifically detects COL4a3 in WB. The antibody is reactive with Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog and Pig samples.

Quick Overview for COL4a3 antibody (Middle Region) (ABIN2788301)

Target: COL4a3 (COL4A3) (Collagen, Type IV, alpha 3 (COL4A3))

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Rabbit, Cow, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL4a3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-COL4a3 Antibody

Binding Specificity: Middle Region

Purpose: COL4A3 Antibody - middle region

Sequence: DPGQPGPPGE QGPPGRCIEG PRGAQGLPGL NGLKGQQGRR GKTGPKGDPG

Predicted Reactivity: Cow: 93%, Dog: 91%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 91%, Pig: 93%, Rabbit: 85%, Rat: 91%

Characteristics: This is a rabbit polyclonal antibody against COL4A3. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human COL4A3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60006-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for COL4a3

Target: COL4a3 (COL4A3) (Collagen, Type IV, alpha 3 (COL4A3))

Alternative Name: COL4A3

Background:

Background Information: Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. There are multiple alternate transcripts that appear to be unique to this human alpha 3 gene and alternate splicing is restricted to the six exons that encode this C-terminal domain. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Some exons of this gene are interspersed with exons of an uncharacterized gene which is on the opposite strand.

Gene Name: Collagen, type IV, alpha 3 (Goodpasture antigen)

Alternative Symbols: ATS2, ATS3

Molecular Weight: 156kDa

Gene ID: 1285

NCBI Accession: NP_112730

UniProt: E7ENN2

Pathways: Sensory Perception of Sound, Positive Regulation of Endopeptidase Activity