GFM1 antibody (C-Term)

Catalog No. ABIN2788619

The Rabbit Polyclonal anti-GFM1 antibody is suitable to detect GFM1 in samples from Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse and Pig. It has been validated for WB.

Quick Overview for GFM1 antibody (C-Term) (ABIN2788619)

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GFM1 Antibody

Binding Specificity: C-Term

Purpose: Gfm1 Antibody - C-terminal region

Sequence: ELRSCTEGKG EYTMEYCRYQ PCSPSTQEEL INKYLEATGQ LPVKKGKAKN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against Gfm1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP60908-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GFM1

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Alternative Name: Gfm1

Background:

Background Information: Gfm1 is a mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Gfm1 catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Gfm1 does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.

Gene Name: G elongation factor, mitochondrial 1

Alternative Symbols: Gfm, AW545374, D3Wsu133, D3Wsu133e

Protein Name: Elongation factor G, mitochondrial

Molecular Weight: 83kDa

Gene ID: 28030

NCBI Accession: NP_613057

UniProt: Q8K0D5