Actin antibody (N-Term)

Catalog No. ABIN2788636

The Rabbit Polyclonal anti-Actin antibody has been validated for WB. It is suitable to detect Actin in samples from Human, Rat, Rabbit, Cow, Guinea Pig and Horse.

Quick Overview for Actin antibody (N-Term) (ABIN2788636)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Rat, Rabbit, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Actin Antibody

Binding Specificity: N-Term

Sequence: GQKDSYVGDE AQSKRGILTL KYPIEHGIIT NWDDMEKIWH HTFYNELRVA

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ACTA1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 377 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: ACTA1

Background: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Alias Symbols: ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3
Protein Interaction Partner: ABL1, TRIM63, TRIM55, CEP57, AMOT, UBC, SUMO3, CDKN2A, ACTG1, BRK1, ACTBL2, NCKAP1, LRCH3, MAPK14, DSTN, NMI, UBL4A, ABRA, ABLIM2, POLR1B, RRN3, HTRA2, PTPN22, ABLIM3, BTK, CCT2, TRAF3IP1, TCP1, SNCA, PARK2, LGALS3, SHC1, GSN, CDK2, CUL1, ISG15, YWHAZ, RE
Protein Size: 377

Molecular Weight: 42 kDa

Gene ID: 58

NCBI Accession: NM_001100, NP_001091

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development