HLA-E antibody (C-Term)

Catalog No. ABIN2789266

This Rabbit Polyclonal antibody specifically detects HLA-E in WB. It exhibits reactivity toward Human, Mouse and Pig.

Quick Overview for HLA-E antibody (C-Term) (ABIN2789266)

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Reactivity: Human, Mouse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-E antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HLA-E Antibody

Binding Specificity: C-Term

Sequence: IPIVGIIAGL VLLGSVVSGA VVAAVIWRKK SSGGKGGSYS KAEWSDSAQG

Predicted Reactivity: Human: 100%, Mouse: 100%, Pig: 92%

Characteristics: This is a rabbit polyclonal antibody against HLA-E. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 358 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HLA-E

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Alternative Name: HLA-E

Background: HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
Alias Symbols: DKFZp686P19218, EA1.2, EA2.1, HLA-6.2, MHC, QA1
Protein Interaction Partner: UBC, CALR, TAP2, UBD, PPP1R16A, KLRD1, KLRC2, CD8A, KLRC1, HLA-E, B2M,
Protein Size: 358

Molecular Weight: 38 kDa

Gene ID: 3133

NCBI Accession: NM_005516, NP_005507

UniProt: P13747

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response