SPHK1 antibody (Middle Region)

Catalog No. ABIN2789510

The Rabbit Polyclonal anti-SPHK1 antibody (ABIN2789510) specifically detects SPHK1 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Goat and Rabbit samples.

Quick Overview for SPHK1 antibody (Middle Region) (ABIN2789510)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Dog, Goat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPHK1 Antibody

Binding Specificity: Middle Region

Purpose: SPHK1 Antibody - middle region

Sequence: DVDLESEKYR RLGEMRFTLG TFLRLAALRT YRGRLAYLPV GRVGSKTPAS

Predicted Reactivity: Cow: 93%, Dog: 93%, Goat: 77%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against SPHK1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP63467-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: SPHK1

Background:

Background Information: The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Name: Sphingosine kinase 1

Alternative Symbols: SPHK

Protein Name: Sphingosine kinase 1

Molecular Weight: 42kDa

Gene ID: 8877

NCBI Accession: NP_001136073

UniProt: Q9NYA1

Pathways: VEGF Signaling