GRAP antibody (N-Term)

Catalog No. ABIN2791566

The Rabbit Polyclonal anti-GRAP antibody is suitable to detect GRAP in samples from Human, Mouse, Rat, Cow, Guinea Pig, Dog, Horse, Rabbit, Goat and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for GRAP antibody (N-Term) (ABIN2791566)

Target: GRAP (GRB2-Related Adaptor Protein (GRAP))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Dog, Horse, Rabbit, Goat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRAP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GRAP Antibody

Binding Specificity: N-Term

Purpose: GRAP Antibody - N-terminal region

Sequence: SDELAFNKGD TLKILNMEDD QNWYKAELRG VEGFIPKNYI RVKPHPWYSG

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against GRAP. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human GRAP

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP70503-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for GRAP

Target: GRAP (GRB2-Related Adaptor Protein (GRAP))

Alternative Name: GRAP

Background:

Background Information: This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.

Alternative Symbols: DFNB114

Protein Name: GRB2-related adapter protein

Molecular Weight: 25kDa

Gene ID: 10750

NCBI Accession: NP_006604

UniProt: Q13588