ASCL1 antibody (Middle Region)

Catalog No. ABIN504560

The Rabbit Polyclonal anti-ASCL1 antibody is suitable to detect ASCL1 in samples from Human, Mouse, Rat, Cow, Guinea Pig and Dog. It has been validated for WB.

Quick Overview for ASCL1 antibody (Middle Region) (ABIN504560)

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASCL1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ASCL1 Antibody

Binding Specificity: Middle Region

Sequence: AGVLSPTISP NYSNDLNSMA GSPVSSYSSD EGSYDPLSPE EQELLDFTNW

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ASCL1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ASCL1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 236 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Alternative Name: ASCL1

Background: ASCL1 may play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. ASCL1 is essential for the generation of olfactory and autonomic neurons. ASCL1 activates transcription by binding to the E box (5'-CANNTG-3').This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: ASH1, HASH1, MASH1, bHLHa46
Protein Interaction Partner: MEF2D, MEF2C, MEF2A, NEUROG2, UBQLN1, EP300, TCF4, BMP2, ASCL1,
Protein Size: 236

Molecular Weight: 25 kDa

Gene ID: 429

NCBI Accession: NM_004316, NP_004307

UniProt: P50553

Pathways: Dopaminergic Neurogenesis