BAX antibody (N-Term)

Catalog No. ABIN2792125

This anti-BAX antibody is a Rabbit Polyclonal antibody detecting BAX in WB and IHC. Suitable for Human, Pig, Cow and Dog. This Primary Antibody has been cited in 2+ publications.

Quick Overview for BAX antibody (N-Term) (ABIN2792125)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Pig, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-BAX Antibody

Binding Specificity: N-Term

Sequence: MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG EAPELALDPV

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Pig: 100%

Characteristics: This is a rabbit polyclonal antibody against BAX. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BAX

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 192 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BAX antibody (ABIN2792125)

De Martino, Marfé, Longo, Fiorito, Montagnaro, Iovane, Decaro, Pagnini: "Bid cleavage, cytochrome c release and caspase activation in canine coronavirus-induced apoptosis." in: Veterinary microbiology, Vol. 141, Issue 1-2, pp. 36-45, (2010) (PubMed).

Honda, Abe, Hiroki, Honda, Iwanuma, Yanagisawa, Ide: "Activation of caspase 3, 9, 12, and Bax in masseter muscle of mdx mice during necrosis." in: Journal of muscle research and cell motility, Vol. 28, Issue 4-5, pp. 243-7, (2007) (PubMed).

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: BAX

Background: BAX encodes a protein that belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. BAX expression is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Alias Symbols: BCL2L4
Protein Interaction Partner: CYCS, ZBTB24, UBC, CASP9, CASP8, CASP7, CASP3, BCL2L1, BCL2, CLU, MDM2, MCL1, BAX, BAG3, YWHAB, VCP, CRYAB, CRYAA, BAK1, XIAP, TP53, KCNA3, HSPA4, XRCC6, XRCC5, HDAC6, BAD, PSMC5, PSMC3, PARK7, UHRF2, RNF144B, OYE2, TOMM22, TOMM40, MEPCE, MOAP1, BBC3, PPP
Protein Size: 192

Molecular Weight: 21 kDa

Gene ID: 581

NCBI Accession: NM_138761, NP_620116

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response