Topoisomerase II alpha antibody (C-Term)

Catalog No. ABIN2792181

Product Details anti-Topoisomerase II alpha Antibody

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Pig, Dog, Rabbit, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VSKAVTSKKS KGESDDFHMD FDSAVAPRAK SVRAKKPIKY LEESDEDDLF

Predicted Reactivity: Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 87%, Pig: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against TOP2A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TOP2A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1531 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A (TOP2A Products)

Background: TOP2A is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
Alias Symbols: TOP2, TP2A
Protein Interaction Partner: UBC, SUMO2, SUMO3, EIF6, RPA3, RPA2, RPA1, SUMO1, LMNA, CTNNB1, ITGA4, FN1, CSNK2A1, VCAM1, SRPK1, RB1, ESR1, TOP2B, CSNK2A2, UPF3B, PRKCG, PRKCB, PRKCA, TERF2, BLM, NEDD8, COPS5, CUL5, FBXW7, ISG15, CUL2, VHL, TCEB1, GSK3B, SUMO4, SMARCAD1, CTCF, RING1,
Protein Size: 1531

Molecular Weight: 174 kDa

Gene ID: 7153

NCBI Accession: NM_001067, NP_001058

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases