VAV1 antibody (Vav 1 Oncogene) (Middle Region)

Details for Product anti-VAV1 Antibody No. ABIN2792554, Supplier: Log in to see
Antigen
  • VAV
  • Vav
  • vav-T
  • vav guanine nucleotide exchange factor 1
  • vav 1 oncogene
  • vav guanine nucleotide exchange factor 1 L homeolog
  • VAV1
  • Vav1
  • vav1.L
Alternatives
anti-Human VAV1 antibody for Western Blotting
Epitope
Middle Region
36
25
25
15
10
9
8
7
6
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
178
83
82
3
3
2
2
1
1
Host
Rabbit
145
35
Clonality
Polyclonal
Conjugate
This VAV1 antibody is un-conjugated
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
155
73
34
15
14
12
8
8
6
2
1
1
Options
Supplier
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human VAV1
Sequence RDRSELSLKE GDIIKILNKK GQQGWWRGEI YGRVGWFPAN YVEEDYSEYC
Cross-Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
Predicted Reactivity Cow: 92%, Dog: 92%, Horse: 92%, Human: 100%, Mouse: 92%, Pig: 92%, Rabbit: 90%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against VAV1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Plasmids, Primers & others Plasmids, Primers & others VAV1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name VAV1 (VAV1 Antibody Abstract)
Background This protein is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication.The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: VAV
Protein Interaction Partner: UBC, CD28, ERBB3, ERBB2, EGFR, PTPN22, SPI1, PLCG1, Mbp, CD5, PDGFRB, NOTCH1, FGFR1, DOK1, TUBB, SH3BP2, CBLB, CBL, FYN, SYK, LCP2, CD19, SOCS1, KHDRBS1, LMNA, TUBA1A, PHAX, ZAP70, ZNF655, DOCK2, XRCC5, PAG1, RACGAP1, MERTK, BLNK, NEK3, AOC3, ARHGDIB, DNM
Protein Size: 845
Molecular Weight 98 kDa
Gene ID 7409
NCBI Accession NM_005428, NP_005419
UniProt P15498
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events
Application Notes WB Suggested Anti-VAV1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: Human heart.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 845 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Vav 1 Oncogene (VAV1) (Middle Region) antibody (ABIN2792554) WB Suggested Anti-VAV1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posit...
Background publications Schneider, Rudd: "CD28 and Grb-2, relative to Gads or Grap, preferentially co-operate with Vav1 in the activation of NFAT/AP-1 transcription." in: Biochemical and biophysical research communications, Vol. 369, Issue 2, pp. 616-21, 2008 (PubMed).

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