SSRP1 antibody (Middle Region)

Catalog No. ABIN2792588

This Rabbit Polyclonal antibody specifically detects SSRP1 in WB. It exhibits reactivity toward Human, Mouse, Rat and Cow.

Quick Overview for SSRP1 antibody (Middle Region) (ABIN2792588)

Target: SSRP1 (Structure Specific Recognition Protein 1 (SSRP1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSRP1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SSRP1 Antibody

Binding Specificity: Middle Region

Purpose: SSRP1 Antibody - middle region

Sequence: MKEYEGGRGE SSKRDKSKKK KKVKVKMEKK STPSRGSSSK SSSRQLSESF

Cross-Reactivity: Cow (Bovine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SSRP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SSRP1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SSRP1

Target: SSRP1 (Structure Specific Recognition Protein 1 (SSRP1))

Alternative Name: SSRP1

Background:

Targeted Subunit: SSRP1

Background Information: SSRP1 is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. SSRP1 contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. It also functions as a co-activator of the transcriptional activator p63. The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Structure specific recognition protein 1

Alternative Symbols: FACT, T160, FACT80

Protein Name: FACT complex subunit SSRP1

Molecular Weight: 81kDa

Gene ID: 6749

NCBI Accession: NP_003137

UniProt: Q08945

Pathways: Chromatin Binding