E2F5 antibody (N-Term)

Catalog No. ABIN184339

This Rabbit Polyclonal antibody specifically detects E2F5 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Rabbit and Zebrafish (Danio rerio).

Quick Overview for E2F5 antibody (N-Term) (ABIN184339)

Target: E2F5

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-E2F5 Antibody

Binding Specificity: N-Term

Sequence: KAEIEDLELK ERELDQQKLL LQQSIKNVMD DSINNRFSYV THEDICNCFN

Cross-Reactivity: Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 84%

Characteristics: This is a rabbit polyclonal antibody against E2F5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human E2F5

Application Details

Application Notes: WB Suggested Anti-E2F5 Antibody Titration: 0.2-1 µg/mL
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 346 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for E2F5

Target: E2F5

Alternative Name: E2F5

Background: E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.
Alias Symbols: E2F-5
Protein Interaction Partner: RBL2, UBC, KMT2D, BRCC3, SMAD3, EP300, XPO1, CREBBP, TFDP1, RBL1, BIRC5, MYC, MT1G,
Protein Size: 346

Molecular Weight: 38 kDa

Gene ID: 1875

NCBI Accession: NM_001951, NP_001942

UniProt: Q15329