E2F1 antibody (pHis357)

Catalog No. ABIN2794309

This Rabbit Polyclonal antibody specifically detects E2F1 in DB. It exhibits reactivity toward Human.

Quick Overview for E2F1 antibody (pHis357) (ABIN2794309)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Dot Blot (DB)

Clone: H357

Product Details anti-E2F1 Antibody

Binding Specificity: pHis357

Purpose: Rabbit Anti-Human Phospho-E2F1(H357) Antibody

Immunogen: This E2F1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding H357 of human E2F1.

Isotype: Ig Fraction

Application Details

Application Notes: Dot Blot
Recommended Dilutions
DB: 1:500
Dot blot analysis of Phospho-hE2F1-H357 Pab on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 μg per ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.260 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background:

Target Description: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Gene Symbol: E2F1

Molecular Weight: 46920 Da

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy