PDK4 antibody (AA 250-277)

Catalog No. ABIN2795139

This anti-PDK4 antibody is a Rabbit Polyclonal antibody detecting PDK4 in WB and IHC. Suitable for Human.

Quick Overview for PDK4 antibody (AA 250-277) (ABIN2795139)

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: E265

Product Details anti-PDK4 Antibody

Binding Specificity: AA 250-277

Purpose: Rabbit Anti-Human PDK4 (E265) Antibody

Immunogen: This PDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-277 amino acids from human PDK4.

Isotype: Ig Fraction

Application Details

Application Notes: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:10-50Western blot analysis of PDK4 Antibody (E265) in CEM cell line lysates (35 μg/lane). PDK4 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4-E265, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C (short-term), -20°C (long-term)

Target Details for PDK4

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Alternative Name: PDK4

Background:

Target Description: PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Gene Symbol: PDK4

Molecular Weight: 46469 Da

Gene ID: 5166

UniProt: Q16654

Pathways: PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect