Nfia/Nf-1 (AA 41-140) antibody (Alexa Fluor 594)

Catalog No. ABIN2804610

Product Details

Target: Nfia/Nf-1

Binding Specificity: AA 41-140

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Alexa Fluor 594

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human NFIA/NF-1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Nfia/Nf-1

Background:

Synonyms: CCAAT binding transcription factor, CCAAT Box Binding Transcription Factor, CCAAT-box-binding transcription factor, CTF, FLJ39164, NF I/A, NF-I/A, NF1 A, NF1-A, NF1A, NFI A, NFI L, NFI-A, NFIA, NFIA_HUMAN, Nuclear Factor 1 A, Nuclear factor 1 A type, Nuclear factor 1 A-type, Nuclear factor 1/A, Nuclear Factor 1A, Nuclear Factor I A, Nuclear factor I/A, nuclear factor I/X CCAAT-binding transcription factor, ynuclear factor I/X CCAAT-binding transcription factor, Nuclear factor I/X, Nuclear Factor IA, TGGCA binding protein, TGGCA-binding protein.

Background: NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, alpha-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.