PKMYT1 antibody (AA 121-220) (Alexa Fluor 594)

Catalog No. ABIN2805392

Product Details anti-PKMYT1 Antibody (Alexa Fluor 594)

Target: PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))

Binding Specificity: AA 121-220

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKMYT1 antibody is conjugated to Alexa Fluor 594

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PKMYT1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PKMYT1

Target: PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))

Alternative Name: PKMYT1 (PKMYT1 Products)

Synonyms: MYT1 antibody, 6230424P17 antibody, AW209059 antibody, Myt1 antibody, RGD1305434 antibody, myt1 antibody, protein kinase, membrane associated tyrosine/threonine 1 antibody, protein kinase, membrane associated tyrosine/threonine 1 S homeolog antibody, PKMYT1 antibody, pkmyt1 antibody, Pkmyt1 antibody, pkmyt1.S antibody

Background:

Synonyms: cdc2 inhibitory kinase, Membrane associated tyrosine and threonine specic cdc2 inhibitory kinase, Membrane-associated tyrosine- and threonine-specic cdc2-inhibitory kinase, MYT1, Myt1 kinase, PKMYT 1, Pkmyt1, PMYT1_HUMAN, Protein kinase membrane associated tyrosine/threonine 1, Protein kinase Myt1, DKFZp547K1610, FLJ20093.

Background: PKMYT1 is a member of the serine/threonine protein kinase family. It preferentially phosphorylates and inactivates cell division cycle 2 protein (cdc2), and thus acts as a negative regulator of entry into mitosis (G2 to M transition). It mediates phosphorylation of cdc2 predominantly on 'Thr-14' and is also involved in Golgi fragmentation. It may be involved in phosphorylation of cdc2 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. It may be a downstream target of Notch signaling pathway during eye development. PKMYT1 is negatively regulated by hyperphosphorylation during mitosis.

Gene ID: 9088

Pathways: Mitotic G1-G1/S Phases, M Phase