HIST1H3A antibody (AA 131-230) (Alexa Fluor 594)

Catalog No. ABIN2814110

Product Details anti-HIST1H3A Antibody (Alexa Fluor 594)

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Binding Specificity: AA 131-230

Reactivity: Human, Mouse, Rat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H3A antibody is conjugated to Alexa Fluor 594

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat, Sheep

Predicted Reactivity: Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HIF3 alpha

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HIST1H3A

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Alternative Name: H3 alpha (HIST1H3A Products)

Synonyms: H3/A antibody, H3FA antibody, histone cluster 1 H3 family member a antibody, histone cluster 1, H3a antibody, HIST1H3A antibody, Hist1h3a antibody

Background:

Synonyms: IPAS, MOP7, PASD7, HIF-3A, bHLHe17, Hypoxia-inducible factor 3-alpha, HIF-3-alpha, HIF3-alpha, Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17, HIF3-alpha-1, Inhibitory PAS domain protein, Member of PAS protein 7, PAS domain-containing protein 7, HIF3A

Background: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea (By similarity).

Gene ID: 64344

UniProt: Q9Y2N7