HK3 antibody (C-Term)

Catalog No. ABIN2842205

The Rabbit Polyclonal anti-HK3 antibody has been validated for WB and IHC (p). It is suitable to detect HK3 in samples from Human and Mouse. There are 2+ publications available.

Quick Overview for HK3 antibody (C-Term) (ABIN2842205)

Target: HK3 (Hexokinase 3 (White Cell) (HK3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB3916

Product Details anti-HK3 Antibody

Binding Specificity: AA 760-789, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This HK3 (Hexokinase III) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 760-789 amino acids from the C-terminal region of human HK3 (Hexokinase III).

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-HK3 antibody (ABIN2842205)

van der Kooij, Rojas-Charry, Givehchi, Wolf, Bueno, Arndt, Tenzer, Mattioni, Treccani, Hasch, Schmeisser, Vianello, Giacomello, Methner: "Chronic social stress disrupts the intracellular redistribution of brain hexokinase 3 induced by shifts in peripheral glucose levels." in: Journal of molecular medicine (Berlin, Germany), Vol. 100, Issue 10, pp. 1441-1453, (2022) (PubMed).

Palmieri, Fitzgerald, Shreeve, Hua, Bronder, Weil, Davis, Stark, Merino, Kurek, Mehdorn, Davis, Steinberg, Meltzer, Aldape, Steeg: "Analyses of resected human brain metastases of breast cancer reveal the association between up-regulation of hexokinase 2 and poor prognosis." in: Molecular cancer research : MCR, Vol. 7, Issue 9, pp. 1438-45, (2009) (PubMed).

Target Details for HK3

Target: HK3 (Hexokinase 3 (White Cell) (HK3))

Alternative Name: HK3 (Hexokinase III)

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Molecular Weight: 99025

Gene ID: 3101

NCBI Accession: NP_002106

UniProt: P52790

Pathways: Carbohydrate Homeostasis, Warburg Effect