HK3 antibody (AA 780-860)

Catalog No. ABIN7221496

This Rabbit Polyclonal antibody specifically detects HK3 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HK3 antibody (AA 780-860) (ABIN7221496)

Target: HK3 (Hexokinase 3 (White Cell) (HK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-HK3 Antibody

Binding Specificity: AA 780-860

Purpose: Rabbit Anti-HXK III Polyclonal Antibody

Specificity: HXK III Polyclonal Antibody detects endogenous levels of HXK III protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human HXK III at AA range: 780-860

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HK3

Target: HK3 (Hexokinase 3 (White Cell) (HK3))

Alternative Name: HXK III

Background: HK3, Hexokinase-3, Hexokinase type III, HK IIIHexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. HK3 encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.

Molecular Weight: 99kD

Gene ID: 3101

UniProt: P52790

Pathways: Carbohydrate Homeostasis, Warburg Effect