Prostate Specific Antigen antibody

Catalog No. ABIN2853579

This anti-Prostate Specific Antigen antibody is a Mouse Monoclonal antibody detecting Prostate Specific Antigen in WB, IHC (p), IHC (fro), EIA and ELISA (Capture). Suitable for Human.

Quick Overview for Prostate Specific Antigen antibody (ABIN2853579)

Target: Prostate Specific Antigen (PSA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prostate Specific Antigen antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), ELISA (Capture)

Clone: CHYH1

Product Details anti-Prostate Specific Antigen Antibody

Specificity: DM1035 reacts with PSA. This antibody is reactive to normal prostate, benign prostate hyperplasia and prostate malignant carcinoma. It has not reacted with other normal tissues tested.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G

Immunogen: Human Prostate Specific Antigen

Isotype: IgG1

Application Details

Application Notes: ELISA: In sandwich ELISA this antibody can be used as a Capture antibody in combinationwith a Monoclonal tracer/detection antibody (HRP conjugate, TMB as substrate). Recommended Capture Coating Dose: 0.5-1.0 μg/well. Under the suggested conditionsapproximately 1 ng/mL of PSA in human serum can be detected with an assay range of0-200 ng/mL. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections (ABC method). Western blot: 10 μg/mL allows visualization of 1 μg/lane human PSA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with double distilled water to a final concentration of 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.0 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Alternative Name: Kallikrein-3 / PSA / KLK3

Background: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates two transcript variants encoding different isoforms. Additional transcript variants have been described, but it is unclear if these transcripts are normally expressed or if they are specific to benign or malignant tumors.Synonyms: Gamma-seminoprotein, P-30 antigen, Prostate-specific antigen, Semenogelase

Gene ID: 354

NCBI Accession: NP_001025218

UniProt: P07288

Pathways: Complement System