Prostate Specific Antigen antibody

Catalog No. ABIN2853633

This Mouse Monoclonal antibody specifically detects Prostate Specific Antigen in WB, IHC (p), IHC (fro) and EIA. It exhibits reactivity toward Human.

Quick Overview for Prostate Specific Antigen antibody (ABIN2853633)

Target: Prostate Specific Antigen (PSA)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prostate Specific Antigen antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: CHYH2

Product Details anti-Prostate Specific Antigen Antibody

Specificity: This antibody detects normal prostate, benign prostate hyperplasia and prostate malignant carcinoma.

Purification: Affinity Chromatography on Protein G

Immunogen: Human Prostate Specific Antigen

Isotype: IgG1

Application Details

Application Notes: ELISA. Western blot: Concentration of 10 μg/mL MAb will allow visualization of 1 μg/lane of humanPSA. Immunohistochemistry-formalin-fixed paraffin embedded and frozen tissue sections usingABC immunoperoxidase assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with double distillated water to a final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.0 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Alternative Name: Kallikrein-3 / PSA / KLK3

Background: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates two transcript variants encoding different isoforms. Additional transcript variants have been described, but it is unclear if these transcripts are normally expressed or if they are specific to benign or malignant tumors.Synonyms: Gamma-seminoprotein, P-30 antigen, Prostate-specific antigen, Semenogelase

Gene ID: 354

NCBI Accession: NP_001025218

UniProt: P07288

Pathways: Complement System