POLG antibody (C-Term)

Catalog No. ABIN2854742

The Rabbit Polyclonal anti-POLG antibody has been validated for WB, ICC, IHC (p), IP and IF. It is suitable to detect POLG in samples from Human.

Quick Overview for POLG antibody (C-Term) (ABIN2854742)

Target: POLG (Polymerase (DNA Directed), gamma (POLG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLG antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-POLG Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Rat

Characteristics: Rabbit Polyclonal antibody to DNA polymerase gamma (polymerase (DNA directed), gamma)
DNA polymerase gamma antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human DNA polymerase gamma. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Rat testis , MCF-7 , MDA-MB-231

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.67 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for POLG

Target: POLG (Polymerase (DNA Directed), gamma (POLG))

Alternative Name: DNA polymerase gamma

Background: Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.

Cellular Localization: Mitochondrion

Molecular Weight: 140 kDa

Gene ID: 5428

UniProt: P54098