Insulin Receptor antibody (C-Term)

Catalog No. ABIN2854945

This Rabbit Polyclonal antibody specifically detects Insulin Receptor in WB, ICC, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Insulin Receptor antibody (C-Term) (ABIN2854945)

Target: Insulin Receptor (INSR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: KO Validated

Product Details anti-Insulin Receptor Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to Insulin Receptor (insulin receptor)
Insulin Receptor antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Insulin Receptor. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , PC-12 , rat liver

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.31 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: insulin receptor

Background: After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Membrane, Single-pass type I membrane protein

Molecular Weight: 156 kDa

Gene ID: 3643

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity