Acetylcholinesterase antibody (C-Term)

Catalog No. ABIN2855111

The Rabbit Polyclonal anti-Acetylcholinesterase antibody has been validated for WB, ICC, IF and IHC (p). It is suitable to detect Acetylcholinesterase in samples from Human, Rat and Mouse.

Quick Overview for Acetylcholinesterase antibody (C-Term) (ABIN2855111)

Target: Acetylcholinesterase (AChE)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Acetylcholinesterase antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Acetylcholinesterase Antibody

Binding Specificity: C-Term

Characteristics: Rabbit Polyclonal antibody to AChE (acetylcholinesterase)
AChE antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human AChE. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Alternative Name: AChE

Background: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Cellular Localization: Cell junction , synapse , Secreted , Cell membrane, Peripheral membrane protein , Isoform T: Nucleus , Isoform H: Cell membrane, Lipid-anchor , GPI-anchor, Extracellular side , Cell junction , synapse , Secreted , Cell membrane , Isoform T: Nucleus , Isof

Molecular Weight: 68 kDa

Gene ID: 43

Pathways: Skeletal Muscle Fiber Development