PIGA antibody

Catalog No. ABIN2855435

The Rabbit Polyclonal anti-PIGA antibody has been validated for WB. It is suitable to detect PIGA in samples from Human.

Quick Overview for PIGA antibody (ABIN2855435)

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIGA Antibody

Cross-Reactivity: Mouse

Characteristics: Rabbit Polyclonal antibody to PIGA (phosphatidylinositol glycan anchor biosynthesis, class A)
PIGA antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PIGA. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.18 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 1 % BSA, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PIGA

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Alternative Name: phosphatidylinositol glycan anchor biosynthesis class A

Background: This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized.

Cellular Localization: Endoplasmic reticulum membrane, Single-pass membrane protein

Molecular Weight: 54 kDa

Gene ID: 5277

UniProt: P37287

Pathways: Inositol Metabolic Process