ATP Synthase B1 (Center) antibody
Reactivity: Human, Mouse
ICC, IF, IHC (p), WB
Host: Rabbit
Polyclonal
unconjugated
-
- Target
- ATP Synthase B1
- Binding Specificity
- Center
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
- Characteristics
-
Rabbit Polyclonal antibody to ATP synthase B1 (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1)
ATP synthase B1 antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human ATP synthase B1. The exact sequence is proprietary.
- Isotype
- IgG
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- Application Notes
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- Comment
-
Positive Control: Molt-4 , mouse brain
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.45 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
- Target
- ATP Synthase B1
- Background
-
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel.
Cellular Localization: Mitochondrion , Mitochondrion inner membrane - Molecular Weight
- 29 kDa
- Gene ID
- 515
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