Melanoma Antigen Family D, 4 (MAGED4) (Center) antibody

Catalog No. ABIN2855734

Product Details

Target: Melanoma Antigen Family D, 4 (MAGED4)

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Characteristics: Rabbit polyclonal antibody to MAGE 1 (melanoma antigen family A, 1 (directs expression of antigen MZ2-E))
MAGE 1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MAGE 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: MCF-7

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details

Target: Melanoma Antigen Family D, 4 (MAGED4)

Alternative Name: MAGE 1 (MAGED4 Products)

Synonyms: MAGE-E1 antibody, MAGE1 antibody, MAGEE1 antibody, melanoma-associated antigen D4 antibody, MAGE family member D4 antibody, LOC100059523 antibody, MAGED4 antibody

Background: This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80 % sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.

Cellular Localization: Cytoplasm

Molecular Weight: 34 kDa

Gene ID: 4100