HSD11B1 antibody (C-Term)

Catalog No. ABIN2855871

This anti-HSD11B1 antibody is a Rabbit Polyclonal antibody detecting HSD11B1 in WB, ICC, IHC (p), IF and IHC (fro). Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for HSD11B1 antibody (C-Term) (ABIN2855871)

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD11B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HSD11B1 Antibody

Binding Specificity: C-Term

Cross-Reactivity: Human, Sheep

Characteristics: Rabbit Polyclonal antibody to HSD11B1 (hydroxysteroid (11-beta) dehydrogenase 1)
HSD11B1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HSD11B1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 40 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-HSD11B1 antibody (ABIN2855871)

Matsumura, Terao, Itami, Katayama: "Local cortisol activation is involved in EGF-induced immunosuppression." in: Dermato-endocrinology, Vol. 9, Issue 1, pp. e1412018, (2017) (PubMed).

Target Details for HSD11B1

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Alternative Name: hydroxysteroid 11-beta dehydrogenase 1

Background: The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.

Cellular Localization: Endoplasmic reticulum membrane

Molecular Weight: 32 kDa

Gene ID: 3290

UniProt: P28845

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Carbohydrate Metabolic Process