GFP antibody

Catalog No. ABIN285794

The Rabbit Polyclonal anti-GFP antibody has been validated for WB and IP. It is suitable to detect GFP in samples from Aequorea victoria. There is 1 publication available.

Quick Overview for GFP antibody (ABIN285794)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-GFP Antibody

Specificity: This antibody neutralizes and binds to human interferon alpha receptor with high affinity. It interacts with extracellular domain and blocks biological action of Type I interferons.

Cross-Reactivity (Details): This antibody reacts with wild -type GFP and it's variants, such as EGFP, EBFP

Purification: purified

Immunogen: GFP antibody was raised in rabbit using E. Coli-expressed full length green fluorescent protein as the immunogen.

Application Details

Application Notes: IP: 1:200-1:500, WB: 1:5,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitue in distilled water.

Concentration: Lot specific

Buffer: Supplied as a lyophilized Potein A purified rabbit IgG with 0.1 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

References for anti-GFP antibody (ABIN285794)

Orozco, Tahirovic, Rentzsch, Schwenk, Haass, Edbauer: "Loss of fused in sarcoma (FUS) promotes pathological Tau splicing." in: EMBO reports, Vol. 13, Issue 8, pp. 759-64, (2012) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression.