Choline Acetyltransferase antibody (N-Term) (Atto 594)

Catalog No. ABIN2869098

The Rabbit Polyclonal anti-Choline Acetyltransferase antibody has been validated for WB, IF and ICC. It is suitable to detect Choline Acetyltransferase in samples from Human.

Quick Overview for Choline Acetyltransferase antibody (N-Term) (Atto 594) (ABIN2869098)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is conjugated to Atto 594

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Choline Acetyltransferase Antibody (Atto 594)

Binding Specificity: N-Term

Specificity: Predicted molecular weight at ~82.5 kDa. Observed molecular weights between 68-70 kDa.

Cross-Reactivity: Human, Mouse

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN2869098 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: Choline Acetyltransferase

Background: Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. ChAT is a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease and Down syndrome (1-3).

Gene ID: 1103

NCBI Accession: NP_001136401

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development