ITGA4 antibody (PE)

Catalog No. ABIN2869980

This Mouse Monoclonal antibody specifically detects ITGA4 in FACS. It exhibits reactivity toward Human, Cow, Dog, Horse, Cat, Sheep and Non-Human Primate.

Quick Overview for ITGA4 antibody (PE) (ABIN2869980)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human, Cow, Dog, Horse, Cat, Sheep, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 9F10

Product Details anti-ITGA4 Antibody (PE)

Purpose: Anti-Hu CD49d PE

Cross-Reactivity (Details): Human, Non-Human Primates, Bovine, Canine (Dog), Equine (Horse), Feline (Cat), Sheep

Characteristics: The mouse monoclonal antibody 9F10 recognizes an extracellular epitope of CD49d (alpha 4 integrin), a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not platelets.

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d

Background: Integrin subunit alpha 4,CD49d / integrin alpha 4, unlike other alpha integrins, neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with beta 7 chain to form alpha 4 / beta 7 integrin, and with beta 1 chain (CD29) to form VLA-4 integrin. These complexes are important for lymphocyte migration from circulation into tissue (binding VCAM-1) and homing of T cell subsets to Peyer´s patches (binding MadCAM-1), but VLA-4 is also target for invasive bacteria which contain invasin. CD49d is essential for differentiation and migration of hematopoietic stem cells by their adhesion to bone marrow stromal cells, and provides a costimulatory signal to TCR-CD3 complex by inducing phosphorylation of some focal adhesion proteins.,ITGA4, VLA-4 alpha

Gene ID: 3676

UniProt: P13612

Pathways: Integrin Complex