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- Target
- CD65
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Flow Cytometry (FACS)
- Specificity
- The mouse monoclonal antibody VIM8 recognizes human CD65, an asialo-fucoganglioside expressed on the surface of peripheral blood granulocytes (highly) and monocytes (weakly).
- Cross-Reactivity (Details)
- Human
- Purification
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- THP-1 cell line
- Clone
- VIM8
- Isotype
- IgM
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anti-CD65 antibody (PE)
Reactivity: Human FACS Host: Mouse Monoclonal VIM8 PE
anti-CD65 antibody (FITC)Reactivity: Human FACS Host: Mouse Monoclonal VIM8 FITC
anti-CD65 antibody (FITC)Reactivity: Human FACS, IF Host: Mouse Monoclonal 88H7 FITC
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- Application Notes
- Flow cytometry: Recommended dilution: 1-5 μg/mL.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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- Target
- CD65
- Background
- CD65 is a fucosylated carbohydrate antigen (ceramide-dodecasaccharide, type II fucoganglioside), which serves as a ligand for CD62E (E-selectin). Its structure is Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide. Unlike CD65s, the CD65 antigen does not contain terminal sialic acid, the rest of their structure is identical. CD65 is expressed on granulocytes and monocytes and participates in cell adhesion. It has been reported as important for extravascular infiltration of acute monocytic leukemia cells.,ceramide-dodecasaccharide, type II fucoganglioside
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