Leptin antibody (Biotin)

Catalog No. ABIN288442

This Rabbit Polyclonal antibody specifically detects Leptin in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for Leptin antibody (Biotin) (ABIN288442)

Target: Leptin (LEP)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leptin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Leptin Antibody (Biotin)

Immunogen: Leptin antibody was raised in rabbit using highly pure recombinant murine leptin as the immunogen.

Application Details

Application Notes: ELISA: 0.25-1 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile PBS with 0.1 % BSA to a concentration of at least 50µg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, pH 7.2.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Background: Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models.

Molecular Weight: 16.2 kDa (predicted detection band MW)

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process