TNF alpha antibody

Catalog No. ABIN289547

The Goat Polyclonal anti-TNF alpha antibody has been validated for WB, ELISA and Inhibition. It is suitable to detect TNF alpha in samples from Human.

Quick Overview for TNF alpha antibody (ABIN289547)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Blocking Antibody (Inhibition)

Product Details anti-TNF alpha Antibody

Purification: Purified

Immunogen: TNF alpha antibody was raised in goat using highly pure recombinant human TNF-alpha as the immunogen.

Isotype: IgG1 kappa

Application Details

Application Notes: ELISA: 0.5-2 µg/mL, Inhibition: 0.012-0.014 µg/mL, WB: 0.1-0.2 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of > 0.2 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from 0.5 x PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C until reconstitution. Following reconstitution product may be stored at 4 °C in the short term. For long term storage aliquot and freeze at -20 °C.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.

Molecular Weight: 17.3 kDa (predicted detection band MW)

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome