UCP2 antibody

Catalog No. ABIN289564

Product Details anti-UCP2 Antibody

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Specificity: This antibody reacts with residues 125-142 EETAKKVRRAIEQLAAMD of the human Survivin protein. Cross reacts with mouse and rat Survivin (88% sequenceidentity).

Cross-Reactivity (Details): This UCP2 peptide sequence is 100 % conserved in rat, hamster, and 86 % in human, pig, canine, and bovine UCP2. It has no significant homology with UCP1, UCP3-5.

Purification: Affinity chromatography purified

Immunogen: UCP2 antibody was raised in rabbit using a 14 amino acid peptide from mouse UCP2 as the immunogen.

Isotype: IgG

Application Details

Application Notes: ELISA: 1:10,000-1:100,000, HC: 2-20 µg/mL, IF: 2-20 µg/mL, WB: 1-10 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in PBS to a concentraiton of 1 mg/mL.

Concentration: Lot specific

Buffer: Affinity purified antibody lyophilized from PBS with 0.1 % BSA.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for UCP2

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Alternative Name: UCP2 (UCP2 Products)

Background: Uncoupling proteins (UCP1-5) are a family of mitochondria transport proteins that play a critical role in thermoregulatory heat production and maintenance of basal metabolic rate. UCP3 is predicted to contain 6 transmembrane domains. The UCP3S lacks the 6th transmembrane domain and it is only 275 aa long. Mouse/rat UCP2 is A 309 AA (human 309 aa chromosome 7, ~95% homology) mitochondrial uncoupling protein. UCP2 has wide tissue distribution in mouse tissues (brain, kidney, liver, brown adipose tissue, heart, and muscle). UCP2 may play a critical role in energy balance, body weight, and thermoregulation.

Pathways: Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Proton Transport