Aurora A antibody (AA 394-403)

Catalog No. ABIN292641

The Goat Polyclonal anti-Aurora A antibody (ABIN292641) specifically detects Aurora A in WB, ELISA, IHC, IHC (p) and IP. The antibody is reactive with Human samples.

Quick Overview for Aurora A antibody (AA 394-403) (ABIN292641)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-Aurora A Antibody

Binding Specificity: AA 394-403

Specificity: Human AURKA / Aurora-A. Reported variants represent identical protein: NP_003591.2, NP_940835.1, NP_940836.1, NP_940837.1, NP_940838.1, NP_940839.1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Marmoset (90%) Sheep, Bovine, Elephant, Panda, Helicobacter (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QNKESASKQS from the C-terminus of human AURKA / Aurora-A (NP_003591.2). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Marmoset (90%), Sheep, Bovine, Elephant, Panda, Helicobacter (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), IP, Peptide-ELISA, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: AURKA / Aurora-A

Background: Name/Gene ID: AURKA
Subfamily: Aurora/IPL1-like
Family: Protein Kinase

Synonyms: AURKA, AIK, AIRK1, ARK1, Aurora-A kinase, Aurora kinase A, Aurora/IPL1-like kinase, AYK1, AURA, AURORA2, Breast tumor-amplified kinase, Breast-tumor-amplified kinase, BTAK, HARK1, IPL1-related kinase, Serine/threonine kinase 6, STK7, STK6, PPP1R47, ARK-1, Aurora 2, Aurora-A, Aurora-related kinase 1, Aurora/IPL1-related kinase 1, IAK1, Serine/threonine kinase 15, STK15

Gene ID: 6790

NCBI Accession: NP_003591

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization