CD97 antibody (AA 220-270)

Catalog No. ABIN292888

This Rabbit Polyclonal antibody specifically detects CD97 in WB, IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for CD97 antibody (AA 220-270) (ABIN292888)

Target: CD97

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD97 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD97 Antibody

Binding Specificity: AA 220-270

Specificity: A portion of amino acids 220-270 of human Leukocyte Antigen CD97.

Purification: Immunoaffinity purified

Immunogen: A portion of amino acids 220-270of human Leukocyte Antigen CD97.

Type of Immunogen: Protein fragment

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD97

Target: CD97

Alternative Name: ADGRE5 / CD97

Background: Name/Gene ID: ADGRE5
Subfamily: CD55
Family: GPCR

Synonyms: ADGRE5, CD97, CD97 Molecule, Leukocyte antigen CD97, TM7LN1, CD97 antigen

Gene ID: 976

UniProt: P48960