GFP antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN294471

This anti-GFP antibody is a Goat Polyclonal antibody detecting GFP in WB, ELISA and IHC. Suitable for Aequorea victoria.

Quick Overview for GFP antibody (Alkaline Phosphatase (AP)) (ABIN294471)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-GFP Antibody (Alkaline Phosphatase (AP))

Specificity: Assay by IEP resulted in a single precipitin arc against anti-goat serum, anti-Alkaline Phosphatase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) serum. No reaction was observed against human, mouse and rat serum proteins.

Purification: Immunoaffinity purified

Immunogen: GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Type of Immunogen: Fusion protein

Application Details

Application Notes: Approved: ELISA (1:16000 - 1:32000), IHC, WB

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:16000-1:32000, 1.0 g of GFP in a standard capture ELISA using pNPP p-nitrophenyl phosphate code as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Aequorea victoria

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50 % glycerol,  pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212