Insulin antibody

Catalog No. ABIN294749

The Mouse Monoclonal anti-Insulin antibody is suitable to detect Insulin in samples from Human. It has been validated for ELISA.

Quick Overview for Insulin antibody (ABIN294749)

Target: Insulin (INS)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: ELISA

Clone: 7D6

Product Details anti-Insulin Antibody

Specificity: The site of connection of C-peptide and C-end of B-chain of proinsulin molecule. MAbs 1D6 and 1D3 react with different epitopes on human proinsulin molecule, both of each are generated by the interactions between insulin and C-peptide parts of prohormone. Kd MAb 3A1 is 1.1 x 10-8 M. Kd MAb 1G4 is 2.6 x 10-7 M. Kd MAb 1D6 and 1D3 is >3 x 10-8 M. Kd MAb 7D6 is >5 x 10-8 M. MAbs 3A1, 1G4, 1D6, 1D3 and 7D6 do not cross-react with human, bovine and porcine insulin, bovine and porcine proinsulin, and human C-peptide.

Purification: Protein A purified

Immunogen: Purified human proinsulin.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background: Name/Gene ID: INS
Family: Hormone

Synonyms: INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin

Gene ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation