TNF alpha antibody

Catalog No. ABIN295308

The Goat Polyclonal anti-TNF alpha antibody (ABIN295308) specifically detects TNF alpha in WB, ELISA and Func. The antibody is reactive with Human samples.

Quick Overview for TNF alpha antibody (ABIN295308)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Product Details anti-TNF alpha Antibody

Specificity: Recognizes human Tumour Necrosis Factor alpha (TNFa€""alpha), a 17.4kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-kappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. TNF-alpha also plays a role in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Purification: Purified

Immunogen: Recombinant human TNF-alpha.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.25 - 1 μg/mL), Func (12 - 14 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: May be used in an indirect ELISA or as a capture reagent in a sandwich ELISA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water. For long term storage the addition of 0.09 % sodium azide is recommended. For functional studies do not add azide.

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.4.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome