Phospholipase C gamma 1 antibody (pTyr783)

Catalog No. ABIN296076

The Rabbit Polyclonal anti-Phospholipase C gamma 1 antibody (ABIN296076) specifically detects Phospholipase C gamma 1 in WB, ELISA, IF and FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for Phospholipase C gamma 1 antibody (pTyr783) (ABIN296076)

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Phospholipase C gamma 1 Antibody

Binding Specificity: pTyr783

Specificity: Recognizes human PLC gamma 1 only when activated by phosphorylation at Tyr783. It does not cross-react with phosphorylated PLC gamma 2 or other PLCs. Species cross-reactivity: Mouse. Species sequence Homology: Rat - 100 % .

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr783 of human PLC gamma 1.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo (1:25), IF, WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry. Western Blot: 1:1000 incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Flow Cytometry: 1:25.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Alternative Name: PLCG1

Background: Name/Gene ID: PLCG1
Family: Phospholipase

Synonyms: PLCG1, Phospholipase C, gamma 1, Phospholipase C-148, Phosphoinositidase C, Phospholipase C-II, Inositoltrisphosphohydrolase, PLC-gamma-1, PLC1, PLC-148, PLC-II, PLC148, NCKAP3, Phospholipase C-gamma-1, PLCgamma1

Gene ID: 5335

UniProt: P19174

Pathways: RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling