Working dilution: Optimal dilution should be determined by the end user. The following are guidelines only : FC(1-2 μg for 10.6 cells) IHC(4 - 5 μg/mL) WB(0.5 - 1 μg/mL)
Positive control: HeLa cell lysate, rat kidney and rat/mouse testes tissue.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.4, glycerol 50 % .
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Dilute in PBS or medium that is identical to that used in the assay system.
Storage
-20 °C
Storage Comment
Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.
FK506-binding proteins, FKBP51 and FKBP52, are high molecular weight immunophilins that are part of the mature glucocorticoid receptor heterocomplex. The N-terminal domains of these proteins bind FK506 and have peptidyl-prolyl isomerase activity that converts prolyl peptide bonds within target proteins from cis- to transproline. The C-terminal domains contain the tetratricopeptide repeats that are involved in protein-protein interactions with Hsp90. Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptors differently and have different Hsp90-binding characteristics. In addition, FKBP51 is linked to the progesterone receptor while FKBP52 is linked to progesterone, androgen, and glucocorticoid receptors.