ROCK2 antibody (pTyr256)

Catalog No. ABIN297208

Product Details anti-ROCK2 Antibody

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Binding Specificity: pTyr256

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: A BLAST analysis was used to suggest cross-reactivity with ROCK-2 from human, mouse, rat, chicken, dog, bovine, Xenopus and Drosophila based on a 100 % homology with the immunizing sequence. Cross-reactivity with ROCK-2 from other sources has not been determined

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:2000 - 1:10000), WB (1:200 - 1:2000)

Usage: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 164 kD in size corresponding to phosphorylated ROCK-2 by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human ROCK-2 pY256 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for ROCK2

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Alternative Name: ROCK2 (ROCK2 Products)

Synonyms: rocka-a antibody, xROK antibody, rock2 antibody, rok2 antibody, ROCK2 antibody, B230113H15Rik antibody, ROKalpha antibody, Rho-kinase antibody, Rock-II antibody, Rock2m antibody, mKIAA0619 antibody, ROCK-II antibody, ROK antibody, Rho-associated, coiled-coil containing protein kinase 2 L homeolog antibody, rho-associated, coiled-coil containing protein kinase 2a antibody, Rho associated coiled-coil containing protein kinase 2 antibody, Rho-associated, coiled-coil containing protein kinase 2 antibody, Rho-associated coiled-coil containing protein kinase 2 antibody, rock2.L antibody, rock2a antibody, ROCK2 antibody, Rock2 antibody

Background: Name/Gene ID: ROCK2
Subfamily: ROCK
Family: Protein Kinase

Synonyms: ROCK2, KIAA0619, p164 ROCK-2, Rho kinase 2, RhoA-associated kinase-2, ROCK-II, Rho kinase II, Rok beta, Rok2, RockII

Gene ID: 9475

UniProt: O75116

Pathways: Microtubule Dynamics, WNT Signaling, Tube Formation